PROJECTS CONDUCTED IN REGENETECH’S LAB

• Development of a specific hematopoietic stem cell counting system of multi-color flowcytometry with ISHAGE strategies
• In vitro expansion of canine peripheral blood and bone marrow hematopoietic stem cells. (3) Evaluation and comparison of Fibroblast and MSC as supporting cells in co-culture with hematopoietic stem cells
• Skin-like structure formed in co-cultures of keratinocytes and fibroblasts as well as keratinocytes and MSC.
• In vitro expansion of human peripheral blood and bone marrow hematopoietic stem cells for the treatment of heart diseases.
• Effects of low oxygen culture on HSC expansion and differentiation: Induction of erythrocyte lineage
• Evaluation of rotation-based bio-reactor in comparison of static culture for HSC ex vivo expansion
• Effects of different culture materials, cytokines and media on HSC expansion and differentiation
• Establishment of Regenetech standard (RGst) methods for the expansion of G-CSF mobilized peripheral blood, cord blood and bone marrow hematopoietic stem cells as well as MSC
• Effects of cell density on rotation-based bio-reactor HSC culture
• Application of Teflon Bag in Rotation culture system
• Comparison of cytokines from different manufacturers in expanding HSC
• Effects of HEPES on the proliferation and differentiation of HSC
• Decreased CD38 expression of HSC in the culture with serum free media
• Effects of TVEMF on the expansion of HSC
• Expansion of unpurified HSC (buffy-coat culture)
• Directed induction of Dendritic cells using in vitro expanded HSC
• Assessment of differentiation states of hematopoietic stem cells following in vitro culture using side and forward scatter of flow cytometry
• Assessment of Aldehyde Dehydrogenase as a marker to be used in stem cell expansion studies
• Effects of RWV and TVEMF culture on gene expression of osteoblasts and chondrocytes: an RT-PCR study
• RWV cultures as a means of growing tumor cells

COLLABORATION PROJECTS IN THIRD-PARTY LABS

• Effects of RWV culture on gene expression of HSC: a microarray study
• Engraftment study of HSC cultured in RWV system
• Gene karyotyping study of HSC cultured in RWV system
• Cord blood HSC culture in RWV system
• Therapeutic effects of TVEMF on healing of bone fracture in rabbits
• Enhancement of immune response to cancer cell inoculation, Influenza virus infection and vaccination in immune-deficient and immune-compromised (including aged) recipients with ex vivo expanded HSC transplantation
• Comparison of engraftment efficiencies of expanded HSC following the cultures with different bio-reactors and different programs

PLANNED OR INITIATED STUDIES

• Directed differentiation of expanded stem cells, including induction of insulin-producing cells, hepatocyte-like cells, neurocytes, immunocytes, etc.
• Tissue construction: skin-like tissue, cartilage, muscles, and more
• Expansion and directed differentiation of ES cells and iPS cells