Regenetech Lab Projects

The following are a sampling of the research projects that have been or are being conducted by Regenetech.

Development of a specific hematopoietic stem cell counting system of multi-color flowcytometry with ISHAGE strategies
In vitro expansion of canine peripheral blood and bone marrow hematopoietic stem cells. (3) Evaluation and comparison of Fibroblast and MSC as supporting cells in co-culture with hematopoietic stem cells
Skin-like structure formed in co-cultures of keratinocytes and fibroblasts as well as keratinocytes and MSC.
In vitro expansion of human peripheral blood and bone marrow hematopoietic stem cells for the treatment of heart diseases.
Effects of low oxygen culture on HSC expansion and differentiation: Induction of erythrocyte lineage
Evaluation of rotation-based bio-reactor in comparison of static culture for HSC ex vivo expansion
Effects of different culture materials, cytokines and media on HSC expansion and differentiation
Establishment of Regenetech standard (RGst) methods for the expansion of G-CSF mobilized peripheral blood, cord blood and bone marrow hematopoietic stem cells as well as MSC
Effects of cell density on rotation-based bio-reactor HSC culture
Application of Teflon Bag in Rotation culture system
Comparison of cytokines from different manufacturers in expanding HSC
Effects of HEPES on the proliferation and differentiation of HSC
Decreased CD38 expression of HSC in the culture with serum free media
Effects of TVEMF on the expansion of HSC
Expansion of unpurified HSC (buffy-coat culture)
Directed induction of Dendritic cells using in vitro expanded HSC
Assessment of differentiation states of hematopoietic stem cells following in vitro culture using side and forward scatter of flow cytometry
Assessment of Aldehyde Dehydrogenase as a marker to be used in stem cell expansion studies
Effects of RWV and TVEMF culture on gene expression of osteoblasts and chondrocytes: an RT-PCR study
RWV cultures as a means of growing tumor cells

Effects of RWV culture on gene expression of HSC: a microarray study
Engraftment study of HSC cultured in RWV system
Gene karyotyping study of HSC cultured in RWV system
Cord blood HSC culture in RWV system
Therapeutic effects of TVEMF on healing of bone fracture in rabbits
Enhancement of immune response to cancer cell inoculation, Influenza virus infection and vaccination in immune-deficient and immune-compromised (including aged) recipients with ex vivo expanded HSC transplantation
Comparison of engraftment efficiencies of expanded HSC following the cultures with different bio-reactors and different programs